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J Bacteriol. 1965 March; 89(3): 675-679
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Effect of Hemin on the Formation of the Cytochrome System of Anaerobically Grown Staphylococcus epidermidis

Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire

ABSTRACT

JACOBS, N. J. (Dartmouth Medical School, Hanover, N.H.), AND S. F. CONTI. Effect of hemin on the formation of the cytochrome system of anaerobically grown Staphylococcus epidermidis. J. Bacteriol. 89:675–679. 1965.—The cytochrome content and respiratory capacity of cells of a strain of Staphylococcus epidermidis grown in complex media under aerobic and anaerobic conditions were determined. Difference spectra revealed cytochromes of the a, b₁, and o types in aerobically grown cells. These cells respired at a rate of 66 µliters of O₂ per hr per mg of cells (dry weight). The cytochrome content of anaerobically grown cells was markedly reduced, and trace amounts only of cytochromes of the b₁ and o type were visible. The respiratory rate, with glucose as substrate, was reduced to about one-tenth of that observed in aerobically grown cells. Cells grown anaerobically in medium supplemented with hemin had as high a content of pigments resembling cytochromes b₁ and o as did aerobically grown cells; the respiratory rate was 60% of that observed in aerobically grown cells. Cytochrome of the a type, similar to that found in aerobically grown cells, was not detected.