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J Bacteriol. 1965 March; 89(3): 725-728
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Replication of Mycoplasma pneumoniae in Broth Culture

Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts

ABSTRACT

LOW, IOLANDA E. (Harvard Medical School, Boston, Mass.), AND MONROE D. EATON. Replication of Mycoplasma pneumoniae in broth culture. J. Bacteriol. 89:725–728. 1965.—Reproducible growth curves of Mycoplasma pneumoniae can be obtained with the use of shaking cultures, which allows earlier and higher yields of the organism than stationary or roller cultures. Decreasing oxygen tension clearly decreases the growth of M. pneumoniae to barely detectable levels, with or without glucose or pyruvate as substrates. The use of phenol red as a pH indicator in the medium is helpful in judging the replication of a culture so that log-phase organisms can be harvested in large amounts.