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J Bacteriol. 1965 March; 89(3): 803-809
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Use of Ligated Segments of Rabbit Small Intestine in Experimental Shigellosis¹

Naval Medical Research Institute, Bethesda, Maryland
Department of Microbiology, University of Maryland, College Park, Maryland

ABSTRACT

ARM, H. G. (Naval Medical Research Institute, Bethesda, Md.), T. M. FLOYD, J. E. FABER, AND J. R. HAYES. Use of ligated segments of rabbit small intestine in experimental shigellosis. J. Bacteriol. 89:803–809. 1965.—Inoculation of ligated segments of small intestine in living rabbits with broth cultures or resting-cell suspensions of recently isolated strains of Shigella caused distension of the segments by accumulation of exudate within 12 hr. Histological changes characteristic of an inflammatory response were similar to those of human bacillary dysentery. Tissue response and accumulation of exudate preceded demonstrable increase in numbers of shigellae inoculated as 2 x 10¹⁰ resting cells. Capability of shigellae to provoke intestinal response was not related to any particular serological group. The active principles concerned with eliciting intestinal response were associated only with preparations containing living organisms. Ability of recently isolated strains to elicit response diminished rapidly during culture on artificial media. The capability was preserved indefinitely by lyophilization soon after isolation from acute bacillary dysentery infections of man. Advantages of using shigellae recently isolated for investigating possible mechanisms of pathogenesis were indicated. During the summer months, the rabbit small intestine was refractory to the activity of shigellae, and positive responses were not observed. Use of ligated segments of rabbit small intestine qualified as an indicator of virulence for the rabbit; and, virulence for the rabbit showed a high degree of correlation with a short period of culture of shigellae on artificial media after isolation from human bacillary dysentery infections.

² Present address: Naval Medical Research Unit No. 2, Taipei, Taiwan.

³ Present address: Naval Preventive Medicine Unit No. 6, Oahu, Hawaii.

¹ From the Bureau of Medicine and Surgery Navy Department, Research Task MR005.09-1100.01. Some of the data in this paper are taken from a dissertation submitted by H. G. Arm to the Faculty of the Graduate School of the University of Maryland in partial fulfillment of the requirements for the Ph.D. degree. A preliminary account was presented at the 62nd Annual Meeting of the American Society for Microbiology, Kansas City, Mo., 6-10 May 1962.

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