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J Bacteriol. 1965 May; 89(5): 1195-1200
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Influence of Aeration and of Pantothenate on Growth Yields of Zymomonas mobilis

Laboratoire de Thermogénèse Biologique, Faculté des Sciences, Marseille, France
Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France

ABSTRACT

BELAÏCH, JEAN-PIERRE (Centre National de la Recherche Scientifique, Marseille, France), AND JACQUES C. SENEZ. Influence of aeration and of pantothenate on growth yields of Zymomonas mobilis. J. Bacteriol. 89:1195–1200. 1965.—The growth yields and rates of Zymomonas mobilis were measured in aerobic and anaerobic cultures on glucose medium containing yeast extract, amino acids, or ammonium chloride as the nitrogen source. In the absence of yeast extract, pantothenate was required. The growth yield and rate of the cultures in synthetic (amino acids) or minimal (NH₄Cl) medium supplemented with pantothenate corresponded only to about one-half the "normal" values obtained in the presence of yeast extract, suggesting a situation of energetically uncoupled growth. Attempts to restore normal growth by the addition of various compounds were unsuccessful. Aeration of the cultures resulted in a partial oxidation of ethyl alcohol to acetate, but did not modify the growth yield nor the division time. Both aerobic and anaerobic cells, however, contained cytochrome c and a cytochrome oxidase of the a₂ type, which was completely inhibited by 10^–4M cyanide. In anaerobically grown cells, an additional cytochrome of the b type was present. The absence of a Pasteur effect suggests that the transfer of electrons by the respiratory chain of Z. mobilis may not be coupled with oxidative phosphorylation. Aeration had no effect on the catalase content of the cells. As shown by C¹⁴-glucose incorporation, 2 to 3% of the glucose metabolized was assimilated by the cells in both synthetic and rich complex medium. No intracellular glycogen nor poly-ß-hydroxybutyrate was accumulated when growth was limited by nitrogen or by phosphate in the presence of excess glucose.

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