This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kelly, M. S. Articles by Pritchard, R. H. Search for Related Content PubMed PubMed Citation Articles by Kelly, M. S. Articles by Pritchard, R. H.

 Previous Article  |  Next Article 

J Bacteriol. 1965 May; 89(5): 1314-1321
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Unstable Linkage Between Genetic Markers in Transformation

^a Medical Research Council Microbial Genetics Research Unit, Hammersmith Hospital, London, England

ABSTRACT

KELLY, M. (Hammersmith Hospital, London, England), AND R. H. PRITCHARD. Unstable linkage between genetic markers in transformation. J. Bacteriol. 89:1314–1321. 1965.—A new type of association of genetic markers in a transformation system has been discovered. Linkage between certain markers is detectable only under particular conditions of deoxyribonucleic acid (DNA) extraction and is sensitive to dilution of the DNA. The data suggest that this unstable association occurs between markers which are on the same molecule but separated by a molecular distance sufficiently great for linkage to be preserved only by mild DNA extraction procedures and at high concentrations of DNA. Linkage can be stabilized by dilution in the presence of a carrier DNA. By use of these unstable linkages, the genetic map of Bacillus subtilis has been extended in both directions from the aromatic gene cluster.

¹ Present address: Department of Microbial Genetics, Karolinska Institute, Stockholm, Sweden.

² Present address: Department of Genetics, University of Leicester, Leicester, England.