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J Bacteriol. 1965 May; 89(5): 1335-1339
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Effect of Various Conditions on Accumulation of Oxytetracycline in Escherichia coli

^a Department of Agricultural Chemistry, University of Tokyo, Tokyo, Japan

ABSTRACT

IZAKI, KAZUO (University of Tokyo, Tokyo, Japan), AND KEI ARIMA. Effect of various conditions on accumulation of oxytetracycline in Escherichia coli. J. Bacteriol. 89:1335–1339. 1965.—Accumulation of large amounts of oxytetracycline occurred in Escherichia coli when the cells were incubated with high concentrations of oxytetracycline (100 to 400 µg/ml) in nutrient broth or in a medium containing glucose, K₂HPO₄, and MgSO₄. In the absence of glucose or MgSO₄, the accumulation was very small. The optimal pH for accumulation was about 6.5. Manganous ion could replace Mg⁺⁺ in promoting the accumulation, though with decreased effectiveness. Malate and succinate were effective promoters of accumulation as well as glucose. Accumulation was inhibited at low temperatures or in the presence of metabolic inhibitors such as 2,4-dinitrophenol or sodium azide.

¹ Present address: Department of Agricultural Biochemistry, Ohio State University, Columbus.