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J Bacteriol. 1965 May; 89(5): 1383-1386
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of a Toxic Moiety of Low Molecular Weight from Clostridium botulinum Type A

Department of Bacteriology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada

ABSTRACT

GERWING, JULIA (The University of British Columbia, Vancouver, B.C., Canada), CLAUDE E. DOLMAN, AND HARDIAL S. BAINS. Isolation and characterization of a toxic moiety of low molecular weight from Clostridium botulinum type A. J. Bacteriol. 89:1383–1386. 1965.—A toxic moiety of low molecular weight has been isolated from a type A strain of Clostridium botulinum, by a method involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose at pH 5.6. By means of electrophoresis and ultracentrifugation, the toxic substance was shown to be homogeneous; a molecular weight of 12,200 was calculated.