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J Bacteriol. 1965 June; 89(6): 1478-1481
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Characterization of Conjugation Factors in Escherichia coli Cell Walls I. Inhibition of Recombination by Cell Walls and Cell Extracts

^a Department of Microbiology, University of Texas, Austin, Texas

ABSTRACT

LANCASTER, JOHN H. (University of Texas, Austin), E. P. GOLDSCHMIDT, AND ORVILLE WYSS. Characterization of conjugation factors in Escherichia coli cell walls. I. Inhibition of recombination by cell walls and cell extracts. J. Bacteriol. 89:1478–1481. 1965.—An assay procedure was devised to determine quantitatively the interference with conjugation of cell-wall fragments from both male and female strains of Escherichia coli. The fertility of the donor is reflected in the assay. Phenol extracts from the cell walls were active, and chemical analysis suggested that the activity resided in the lipopolysaccharide fraction.

¹ Present address: Department of Botany and Microbiology, University of Oklahoma, Norman.

² Present address: Department of Molecular Biology, M.D. Anderson Hospital and Tumor Institute, Houston, Tex.