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J Bacteriol. 1965 June; 89(6): 1521-1525
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Amino Acid Utilization by Alcaligenes viscolactis for Growth and Slime Production¹

^a Department of Dairy Industries, University of Minnesota, St. Paul and Minneapolis, Minnesota
^b Department of Animal Husbandry, University of Minnesota, St. Paul and Minneapolis, Minnesota
^c Department of Microbiology, University of Minnesota, St. Paul and Minneapolis, Minnesota

ABSTRACT

PUNCH, J. D. (University of Minnesota, St. Paul), J. C. OLSON, JR., AND J. V. SCALETTI. Amino acid utilization by Alcaligenes viscolactis for growth and slime production. J. Bacteriol. 89:1521–1525. 1965.—The ability of Alcaligenes viscolactis to utilize amino acids in a basal salts solution (K₂HPO₄, KH₂PO₄, MgSO₄, MnSO₄, FeSO₄, NaCl) was studied. Of 27 amino acids, only L-asparagine, L-glutamic acid, L-aspartic acid, L-glutamine, L-proline, and L-tyrosine supported growth sufficient to give a viscous supernatant solution. L-Proline and L-tyrosine, singly or combined, fulfilled the carbon, nitrogen, and energy requirements for growth and slime production. None of eight inorganic nitrogenous compounds supported growth with lactose as the carbon source. The addition of L-asparagine, L-aspartic acid, or L-glutamine to L-tyrosine or L-proline, singly or combined, did not increase growth or slime production, indicating no nutritional interactions among these amino acids. Neither lactose nor glucose was found to be required or utilized by A. viscolactis in a medium containing basal salts, L-proline, L-tyrosine, and lactose or glucose. This was established by the fact that total carbohydrate and total reducing activity remained constant during growth and slime production.

² Present address: Department of Microbiology, Medical School, University of New Mexico, Albuquerque.

¹ Scientific Journal Series Paper No. 5399, Minnesota Agricultural Experiment Station, St. Paul.