This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Johnson, T. C. Articles by McLaren, L. C. Search for Related Content PubMed PubMed Citation Articles by Johnson, T. C. Articles by McLaren, L. C.

Next Article 

J Bacteriol. 1965 September; 90(3): 565-570
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Plaque Development and Induction of Interferon Synthesis by RMC Poliovirus

^a Department of Microbiology, University of Minnesota, Minneapolis, Minnesota

ABSTRACT

JOHNSON, TERRY C. (University of Minnesota, Minneapolis), AND LEROY C. MCLAREN. Plaque development and induction of interferon synthesis by RMC poliovirus. J. Bacteriol. 90:565–570. 1965.—Plaque development by RMC poliovirus on human amnion cell monolayers was investigated with regard to autointerference and to the effect of acid-agar overlay on plaquing efficiency. The virus was inhibited by acid-agar overlay, thereby exhibiting the d^– marker typical of attenuated poliovirus strains. In addition, a lack of RMC poliovirus plaque development on HeLa cell monolayers was shown to be the result of an agar inhibitor which could be removed by NaCl extraction. By use of a simplified plaque reduction assay, it was shown that interferon production was responsible for the autointerference phenomenon. Interferon synthesis did not correlate with the ages in vitro of human amnion cell cultures. Fibroblasts originating from the chorionic membrane produced negligible amounts of the inhibitor. Interferon synthesis by human amnion cells infected with RMC poliovirus was inhibited by actinomycin D. The addition of guanidine hydrochloride to infected cultures immediately after RMC poliovirus adsorption markedly inhibited interferon synthesis, although after 2 hr (postadsorption) guanidine had no effect on interferon production.

¹ Present address: Department of Molecular and Cell Biology, University of California, Irvine.

² Present address: Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque.