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J Bacteriol. 1965 September; 90(3): 643-647
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Relative Contribution of the Cell Wall, Cytoplasmic Membrane, and Cytoplasm to the Gram-Positive Characteristic of Bacillus megaterium

Microbiology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California

ABSTRACT

BARTHOLOMEW, J. W. (University of Southern California, Los Angeles), AND THOMAS CROMWELL. Relative contribution of the cell wall, cytoplasmic membrane, and cytoplasm to the gram-positive characteristic of Bacillus megaterium. J. Bacteriol. 90:643–647. 1965.—A comparison of the roles of the cell wall, cytoplasmic membrane, and cytoplasmic components revealed that the intact cell wall was the dominant contributor to the gram-positive state. Protoplasts of Bacillus megaterium were confirmed as being gram-negative, as reported by Gerhardt et al. The "gram-positive protoplast" report of Amano et al. was shown to be a laboratory-produced artifact, resulting from the comparison of smears made from saline suspensions of Escherichia coli cells with smears made from formalin-sucrose suspensions of B. megaterium protoplasts.