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J Bacteriol. 1965 September; 90(3): 667-672
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Extraction of Cell-Wall Polysaccharide Antigen from Streptococci

Hutton D. Slade1

a Department of Microbiology, Northwestern University Medical School, Chicago, Illinois, and Max-Planck Institut für Immunbiologie, Freiburg, Germany

ABSTRACT

SLADE, HUTTON D. (Northwestern University Medical School, Chicago, Ill., and Max-Planck Institut für Immunbiologie, Freiburg, Germany). Extraction of cell-wall polysaccharide antigen from streptococci. J. Bacteriol. 90:667–672. 1965.—The carbohydrate grouping antigens in the cell walls of streptococci belonging to groups A, E, G, L, and T were extracted with 5% trichloroacetic acid at 90 C. The antigens were removed also from dry whole cells by extraction with trichloroacetic acid followed by treatment with phenol-water. Details of the methods are presented. The antigens obtained by use of either of these procedures were suitable for studies on immunological specificity and chemical structure. Quantitative enzymatic and chemical analyses of two group E antigens and one group T preparation showed the presence of L-rhamnose (22 to 44%), D-glucose (7 to 22%), D-galactose (T antigen only, 26%), glucosamine (2 to 16%), and galactosamine (T antigen only, 3%). In addition, analyses of A and G antigen preparations are presented. The protein and phosphate content of the A and E antigens were about 1% each. Quantitative precipitin curves of these antigens are presented.

FOOTNOTES

1 Public Health Service Research Career Awardee (K6-GM-16284) of the Division of General Medical Sciences, National Institutes of Health.

J Bacteriol. 1965 September; 90(3): 667-672
Copyright © 1965 American Society for Microbiology. All Rights Reserved.





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