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J Bacteriol. 1965 September; 90(3): 710-714
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Influence of Inoculum Media on Sensitivity of Escherichia coli to Azaserine¹

^a Kettering-Meyer Laboratory,² Southern Research Institute, Birmingham, Alabama

ABSTRACT

NARKATES, ANNIE J. (Southern Research Institute, Birmingham, Ala.), AND ROBERT F. PITTILLO. Influence of inoculum media on sensitivity of Escherichia coli to azaserine. J. Bacteriol. 90:710–714. 1965.—Escherichia coli grown in a simple synthetic medium is more than 100-fold more sensitive to inhibition by azaserine than E. coli grown in complex, undefined organic medium. The work reported here offers two explanations for this observed effect: (i) concentration within the cell from the complex medium of a specific azaserine-reversing metabolite (nicotinic acid), and (ii) stimulation by exogenous metabolite(s) of intracellular synthesis of azaserine-reversing agents.

² Affiliated with Sloan-Kettering Institute for Cancer Research, New York, N.Y.

¹ A preliminary report of this investigation was presented at the Annual Meeting of the American Society for Microbiology, Washington, D.C., 3–7 May 1964.