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J Bacteriol. 1965 October; 90(4): 1120-1128
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Immunochemical Studies on -Amylase III. Immunochemical Relationships Among Amylases from Various Microorganisms¹

^a Department of Dental Research, University of Rochester School of Medicine and Dentistry, Rochester, New York
^b Department of Microbiology, University of Rochester School of Medicine and Dentistry, Rochester, New York

ABSTRACT

SIRISHINHA, STITAYA (University of Rochester School of Medicine and Dentistry, Rochester, N.Y.), AND PETER Z. ALLEN. Immunochemical studies on -amylase. III. Immunochemical relationships among amylases from various microorganisms. J. Bacteriol. 90:1120–1128. 1965.—Immunochemical relationships among amylases obtained from a selected group of microorganisms were examined, and a cross-reaction was detected between the -amylases of Bacillus stearothermophilus and B. subtilis. Immunodiffusion and quantitative precipitin studies, as well as cross-neutralization tests, indicate that B. stearothermophilus -amylase reacts with a portion of antibody present in antisera to crystalline B. subtilis -amylase. Amylases from these two species thus have some aspects of structure in common. Limited data obtained by immunodiffusion suggest that groupings which confer cross-reactivity to the B. stearothermophilus enzyme are lost after exposure to mercaptoethanol in the presence of ethylenediamine-tetraacetate, followed by treatment with iodoacetamide. With the antisera employed and within the concentration range examined, no immunochemical cross-reaction was observed among amylases from Aspergillus oryzae, B. subtilis, B. polymyxa, B. macerans, Pseudomonas saccharophila, and Euglena sanguinis. Immunoelectrophoresis of partially purified B. stearothermophilus -amylase by use of antiserum to the crude enzyme, together with localization of amylase activity in immunoelectrophoretic plates, suggests that B. stearothermophilus -amylase is antigenic in the rabbit.

¹ A portion of this investigation has been submitted to the University of Rochester School of Medicine and Dentistry by Stitaya Sirisinha in partial fulfillment for the requirements for the Ph.D. degree.