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J Bacteriol. 1965 October; 90(4): 911-920
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Development of Competence of Haemophilus influenzae

^a Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland

ABSTRACT

SPENCER, HUGH T. (The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Md.), AND ROGER M. HERRIOTT. Development of competence of Haemophilus influenzae. J. Bacteriol. 90:911–920. 1965.—A chemically defined nongrowth medium was developed for the induction of competence of Haemophilus influenzae by a stepdown procedure. Cells grown logarithmically in Heart Infusion Broth became competent after being transferred to a medium which consisted of amino acids, sodium fumarate, and inorganic salts. Chloramphenicol (2 µg/ml) or L-valine (1 µg/ml) in the nongrowth medium inhibited development of competence. The inhibitory action of L-valine was reversed by comparable concentrations of L-isoleucine. Kinetic studies of the development of competence showed a variable capacity of competent cells to take up deoxyribonucleic acid and reaffirmed earlier findings that competence was not transmissible in H. influenzae. Addition of nicotinamide adenine dinucleotide, thiamine, calcium pantothenate, uracil, and hypoxanthine to the medium for competence resulted in a minimal growth medium in which reduced levels of competence were developed.

¹ Present address: Department of Biochemistry, University of Tennessee, Knoxville.

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