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J Bacteriol. 1965 December; 90(6): 1710-1712
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Nitrogen Dioxide Fixation in Bacterial Chromatin Studies¹

^a Biology Department, St. John's University, Jamaica, New York

ABSTRACT

ELLER, CHARLES (St. John's University, Jamaica, N.Y.), AND WILLIAM H. BECKERT. Nitrogen dioxide fixation in bacterial chromatin studies. J. Bacteriol. 90:1710–1712. 1965.—Satisfactory chromatin staining was obtained with two Bacillus species when nitrogen dioxide was used for fixation in conjunction with either the acid Giemsa or Feulgen staining methods. An impression method was employed for the transfer of bacilli to a new environment as well as for smears. Advantages of nitrogen dioxide fixation are discussed.

² Present address: Microbiology Section, U.S. Air Force School of Aerospace Medicine, Brooks Air Force Base, Tex.

¹ Taken in part from a thesis submitted by the senior author in partial fulfillment of the requirements for the M.S. degree from St. John's University.