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J Bacteriol. 1966 December; 92(6): 1618-1622
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Kinetics of Fungal Growth and Phosphatase Formation in Aspergillus nidulans

Department of Genetics, Albert Einstein College of Medicine, New York, New York

ABSTRACT

DORN, G. (Albert Einstein College of Medicine, New York, N.Y.), AND W. RIVERA. Kinetics of fungal growth and phosphatase formation in Aspergillus nidulans. J. Bacteriol. 92:1618–1622. 1966.—A liquid culture system is described for growing Aspergillus nidulans in a manner amenable to kinetic studies. Changes in dry weight, protein, deoxyribonucleic acid (DNA), inorganic phosphate, alkaline phosphatase, and acid phosphatase activity were followed over a 40-hr interval. In both limiting- and high-phosphate media, the doubling time of DNA during the logarithmic phase was 3.5 ± 0.5 hr. Of the four phosphatases which have been found in this fungus, three were markedly derepressed by low levels of inorganic phosphate.