This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Freda, C. E. Articles by Cohen, S. S. Search for Related Content PubMed PubMed Citation Articles by Freda, C. E. Articles by Cohen, S. S.

 Previous Article  |  Next Article 

J Bacteriol. 1966 December; 92(6): 1680-1688
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Nature of Ribonucleic Acid Stimulated by Streptomycin in the Absence of Protein Synthesis¹

^a Department of Therapeutic Research, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania

ABSTRACT

FREDA, CELIA E. (University of Pennsylvania School of Medicine, Philadelphia), AND SEYMOUR S. COHEN. Nature of ribonucleic acid stimulated by streptomycin in the absence of protein synthesis. J. Bacteriol. 92:1680–1688. 1966.—The ribonucleic acid (RNA) synthesized in a thymineless, arginineless, uracil-less Escherichia coli strain 15 in the absence of arginine was characterized by sucrose density gradient centrifugation. About 60% of this RNA had sedimentation rates in the range between 4S and 16S, and the remainder was comprised of the 23S and 16S ribosomal components. On addition of streptomycin for 1 hr in the absence of the amino acid, there was an inhibition of synthesis of material of 4S to 16S, whereas 16S RNA was slightly stimulated. Between 1 and 3 hr after addition of the antibiotic, during the precipitous killing of the bacteria in the arginine-deficient culture, the synthesis of 16S ribosomal RNA was specifically and sharply stimulated.

¹ The data in this paper are taken from a dissertation presented by Celia E. Freda to the faculty of the Graduate School of Arts and Sciences of the University of Pennsylvania in partial fulfillment of the requirements for the Ph.D. degree.