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J Bacteriol. 1966 December; 92(6): 1710-1715
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Enhancement of Rhinovirus Plaque Formation in Human Heteroploid Cell Cultures by Magnesium and Calcium¹

^a Department of Preventive Medicine, University of Washington School of Medicine, Seattle, Washington

ABSTRACT

FIALA, MILAN (University of Washington, Seattle), AND GEORGE E. KENNY. Enhancement of rhinovirus plaque formation in human heteroploid cell cultures by magnesium and calcium. J. Bacteriol. 92:1710–1715. 1966.—A reproducible macroplaque assay for six M and three H strains of rhinoviruses has been developed in several human heteroploid cell lines. Plaques were produced only with suitable solidifying agents: purified agar (Ionagar, Agarose) or methylcellulose. Plaque development was greatly enhanced by increasing Mg⁺² to 30 to 40 mM. Diethylaminoethyl (DEAE) dextran also increased plaque sizes, and the effects of Mg⁺² and DEAE dextran were additive. In addition, Ca⁺² substituted for Mg⁺². The suitability of human heteroploid cell lines for rhinovirus plaque assay varied greatly, ranging from insensitivity through partial to complete sensitivity. This assay was six to seven times more sensitive than an end point tube assay. These results indicate that potentiation of plaque formation by Mg⁺² known for some enteroviruses can also be extended to the rhinovirus group of picornaviruses.

² Present address: The John Herr Musser Department of Research Medicine, Hospital of the University of Pennsylvania, Philadelphia.

¹ Some of the data in this paper were presented in preliminary communications: Clin. Res. 14:143, 1966; Federation Proc. 25:492, 1966. This paper represents a thesis submitted by Milan Fiala in partial fulfillment of the requirements for the M.S. degree in Preventive Medicine, University of Washington, Seattle.

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