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J Bacteriol. 1967 January; 93(1): 122-124
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

X-Ray Diffraction Studies on Selected Bacterial Cell Walls

U.S. Naval Applied Science Laboratory, Brooklyn, New York

ABSTRACT

The cell walls of selected bacteria were studied by X-ray diffraction analysis to determine and characterize crystalline components. The walls were isolated by mechanical disruption and purified by enzymatic and washing procedures. The X-ray diffraction lines which appeared from the gram-positive cell walls were shown to be due to the constituent "mucopeptide" fraction. No diffraction lines could be obtained from the gram-negative bacterium studied. The results show that crystallinity is associated with mucopeptide.