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Utilization of 5-Bromouracil by Thymineless Bacteria

^a Sections of Radiation Biology and Genetics, Department of Biology, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas

ABSTRACT

Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an "absolute" thymine requirement could be easily distinguished from one with a "leaky" thymine requirement.

¹ Postdoctoral Fellow of the American Cancer Society (PF-277).

² Faculty Research Associate of the American Cancer Society (PRA-19).

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