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J Bacteriol. 1967 February; 93(2): 525-530
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Identification of Staphylococcal Hemolysins by an Electrophoretic Localization Technique

Department of Microbiology, University of Illinois Medical Center, Chicago, Illinois

ABSTRACT

A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The -hemolysin produced by a White variant of the Wood 46 strain of Staphylococcus aureus migrated 18 mm towards the cathode, and it lysed horse, rabbit, and sheep erythrocytes, whereas a Clear variant of the Wood 46 strain of S. aureus produced a lysin which migrated similarly to the -hemolysin but lysed only rabbit cells. This latter lysin was tentatively named ₁-lysin. This strain of S. aureus also produced ß-hemolysin which migrated 36 mm towards the cathode and lysed sheep cells. ß-Hemolysin produced by some strains of S. aureus showed considerable tailing during electrophoresis, whereas ß-hemolysin produced by other strains of S. aureus migrated as a well-defined peak. A lysin migrating 11 mm towards the anode was probably -lysin. It was, however, not produced in sufficient concentration when the cultures were grown in semisolid medium.

This article has been cited by other articles:

• Bernheimer, A. W. (1968). Cytolytic Toxins of Bacterial Origin: The nature and properties of cytolytic proteins are discussed with emphasis on staphylococcal agr-toxin. Science 159: 847-851