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Isolation and Characterization of a Recombination-Deficient Hfr Strain

^a Massachusetts Institute of Technology, Cambridge, Massachusetts

ABSTRACT

The isolation of a rec^– Hfr strain of Escherichia coli K-12 is described. The method used consisted of mating AB2463 F^– Rec^– His^– Lac^– with P4X6 Hfr Rec⁺ His⁺ Lac⁺, selecting Rec^– His^– Lac⁺ recombinants, and searching for Hfr strains. One Hfr rec^– strain, no. 12, was used as donor in crosses with Rec⁺ and Rec^– recipients. Crosses with Rec⁺ recipients are fertile, and those with Rec^– recipients are almost infertile, the frequency of recombinants being 10^–2 to 10^–3 that found with Rec⁺ recipients. The Rec^– mutant marker is transfered to and integrated into Rec⁺ recipients. Zygotic induction of prophage is observed in crosses between two Rec^– strains. In crosses of F^– Rec^– with Hfr Rec^–, the gradient of integration frequencies for markers progressively more distant from the origin is steeper than in the Rec⁺ x Rec^– or the Rec^– x Rec⁺ crosses.

¹ Present address: Israel Institute of Biological Research, Ness-Ziona, Israel.

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