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Effects of Valine on Protein Synthesis and Turnover in Pseudomonas saccharophila under "Nongratuitous" Inducing Conditions

Exobiology Division, Ames Research Center, National Aeronautics and Space Administration, Moffett Field, California

ABSTRACT

Under "nongratuitous" inducing conditions, in Pseudomonas saccharophila, D-and L-valine and L-isoleucine inhibit net protein synthesis. At a concentration of 0.5 µmole or greater of valine per mg of bacterial protein, net protein synthesis declined approximately 70%. The inhibitory effect of valine is proportional to the exogenous valine concentration. Studies of ¹⁴C amino acid incorporation and ¹⁴C amino acid release from prelabeled cells indicate that valine stimulates protein turnover.