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J Bacteriol. 1967 April; 93(4): 1281-1288
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Phosphate Uptake in an Obligately Marine Fungus II. Role of Culture Conditions, Energy Sources, and Inhibitors

^a Biology Department, Brooklyn College of the City University of New York, Brooklyn, New York

ABSTRACT

Phosphate uptake in the obligately marine fungus, Thraustochytrium roseum, is maximal at pH 7.5 to 7.8, is dependent on temperature, and varies with phosphate concentration. Pyruvate and succinate stimulate phosphate uptake, although they do not increase respiration. The uncoupling agents, 2,4-dinitrophenol and dicoumerol, inhibit phosphate uptake but stimulate oxygen consumption only in the presence of NaCl. Oligomycin inhibits both processes. Among the inhibitors of protein synthesis, chloramphenicol reduces phosphate uptake without affecting respiration. Puromycin is unique in that it greatly enhances phosphate uptake and abolished the lag period associated with this phenomenon. It does not affect respiration.

¹ Present address: Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, Neuchâtel, Switzerland.