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Glucose-Lactose Diauxie in Escherichia coli

^a Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts

ABSTRACT

Growth of Escherichia coli in medium containing glucose, at a concentration insufficient to support full growth, and containing lactose, is diauxic. A mutation in the gene, CR, which determines catabolite repression specific to the lac operon, was found to relieve glucose-lactose but not glucose-maltose diauxie. Furthermore, a high concentration of lactose was shown to overcome diauxie in a CR⁺ strain. Studies on the induction of ß-galactosidase by lactose suggested that glucose inhibits induction by 10^–2M lactose. Preinduction of the lac operon was found to overcome this effect. The ability of glucose to prevent expression of the lac operon by reducing the internal concentration of inducer as well as by catabolite repression is discussed.

¹ Present address: Department of Biology, University of California in San Diego, La Jolla.

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