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Genetic Analysis of Glutamate Transport and Glutamate Decarboxylase in Escherichia coli¹

^a Department of Bacteriology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

ABSTRACT

The location of the Escherichia coli K-12 genes determining or regulating glutamate transport, and the location of the gene determining glutamate decarboxylase synthesis, were established by conjugation. The ability to grow on glutamate as the sole source of carbon and energy was used to select for glutamate transport recombinants. Two genes determining the ability to grow on glutamate as the sole source of carbon and energy were mapped. One (gltC) is located near mtl (mannitol), and the other (gltH) appears to be located between the gal (galactose) and trp (tryptophan) loci. The glutamate decarboxylase gene (gad) is strongly linked to gltC. The gltC⁺ recombinants grow on glutamate much faster and accumulate this amino acid to a greater extent than do the gltH⁺ recombinants. The gltH⁺ gene functioned only in one female strain (P678), whereas the gltC gene functioned in all the female strains tested (P678, C600, W1).

¹ Part of this work was taken from the Ph.D. thesis to be submitted by M. M. to the Senate of the Hebrew University.

This article has been cited by other articles:

• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]