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Genetic Mapping of Mutations Affecting Phosphoglucose Isomerase and Fructose Diphosphatase in Escherichia coli

¹ Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT

The loci on the Escherichia coli genome of mutations affecting phosphoglucose isomerase (pgi) and fructose diphosphatase (fdp) have been determined by conjugation and transduction experiments, chiefly three-factor crosses. The loci for these two constitutive enzymes of central intermediary metabolism lie in the same region of the chromosome, but they are not cotransducible. The order of some markers in this region of the chromosome is arg(ACFH) metA pgi malB uvrA fdp pyrB thr.

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