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Carbohydrate Metabolism in Leukocytes VII. Metabolism of Glucose, Acetate, and Propionate by Human Plasma Cells

¹ Department of Biochemistry, Western Reserve University, Cleveland, Ohio 44106

ABSTRACT

Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with ¹⁴C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 µmole per hr per 10⁸ cells and was mainly via the Embden-Meyerhof pathway, and only 6% or less traversed the hexose monophosphate shunt. The presence of Krebs cycle activity was demonstrated by direct isolation of several labeled intermediates after incubation with either ¹⁴C-acetate or ¹⁴C-propionate. The distribution of ¹⁴C in lactate, succinate, fumarate, malate, aspartate, and glutamate indicate a complete Krebs cycle. Acetate was metabolized via the Krebs cycle to the extent of 0.15 µmoles per hr per 10⁸ cells, and the rate of propionate utilization was 0.17 µmoles per hr per 10⁸ cells.

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