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Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast

Department of Molecular and Cell Biology, University of California, Irvine, California 92650

ABSTRACT

Approximately 400 temperature-sensitive mutants of Saccharomyces cerevisiae were isolated. The mutants were unable to form colonies on enriched media at 36 C, but grew normally, or nearly so, at 23 C. The mutants were tested for loss of viability, change in morphology, increase in cell number, and the ability to synthesize protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA) after a shift from 23 to 36 C. Mutations were found which resulted in a preferential loss of ability to carry out protein synthesis, RNA synthesis, DNA synthesis, cell division, or cell-wall formation. Diploid cells heterozygous for the temperature-sensitive mutations were constructed and tested for their ability to form colonies at 36 C. Four mutations dominant to their wild-type allele were identified.

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• Osborn, M. J., Miller, J. R. (2007). Rescuing yeast mutants with human genes. Brief Funct Genomic Proteomic 0: elm017v1-8 [Abstract] [Full Text]  
• Mitra, D., Parnell, E. J., Landon, J. W., Yu, Y., Stillman, D. J. (2006). SWI/SNF Binding to the HO Promoter Requires Histone Acetylation and Stimulates TATA-Binding Protein Recruitment.. Mol. Cell. Biol. 26: 4095-4110 [Abstract] [Full Text]  
• GRAINGER, R. J., BEGGS, J. D. (2005). Prp8 protein: At the heart of the spliceosome. RNA 11: 533-557 [Abstract] [Full Text]  
• Hossain, M. S, Kurokawa, K., Akimitsu, N., Sekimizu, K. (2004). DNA topoisomerase II is required for the G0-to-S phase transition in Drosophila Schneider cells, but not in yeast. GENES CELLS 9: 905-917 [Abstract] [Full Text]  
• Pessoa-Brandao, L., Sclafani, R. A. (2004). CDC7/DBF4 Functions in the Translesion Synthesis Branch of the RAD6 Epistasis Group in Saccharomyces cerevisiae. Genetics 167: 1597-1610 [Abstract] [Full Text]  
• Seewald, M. J., Kraemer, A., Farkasovsky, M., Korner, C., Wittinghofer, A., Vetter, I. R. (2003). Biochemical Characterization of the Ran-RanBP1-RanGAP System: Are RanBP Proteins and the Acidic Tail of RanGAP Required for the Ran-RanGAP GTPase Reaction?. Mol. Cell. Biol. 23: 8124-8136 [Abstract] [Full Text]  
• McCleland, M. L., Gardner, R. D., Kallio, M. J., Daum, J. R., Gorbsky, G. J., Burke, D. J., Stukenberg, P. T. (2003). The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 17: 101-114 [Abstract] [Full Text]  
• Winzeler, E. A., Castillo-Davis, C. I., Oshiro, G., Liang, D., Richards, D. R., Zhou, Y., Hartl, D. L. (2003). Genetic Diversity in Yeast Assessed With Whole-Genome Oligonucleotide Arrays. Genetics 163: 79-89 [Abstract] [Full Text]  
• Formosa, T., Ruone, S., Adams, M. D., Olsen, A. E., Eriksson, P., Yu, Y., Rhoades, A. R., Kaufman, P. D., Stillman, D. J. (2002). Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae Cause Dependence on the Hir/Hpc Pathway: Polymerase Passage May Degrade Chromatin Structure. Genetics 162: 1557-1571 [Abstract] [Full Text]  
• Porter, S. E., Washburn, T. M., Chang, M., Jaehning, J. A. (2002). The Yeast Paf1-RNA Polymerase II Complex Is Required for Full Expression of a Subset of Cell Cycle-Regulated Genes. Eukaryot Cell 1: 830-842 [Abstract] [Full Text]  
• Harkness, T. A. A., Davies, G. F., Ramaswamy, V., Arnason, T. G. (2002). The Ubiquitin-Dependent Targeting Pathway in Saccharomyces cerevisiae Plays a Critical Role in Multiple Chromatin Assembly Regulatory Steps. Genetics 162: 615-632 [Abstract] [Full Text]  
• McDonald, H. B., Helfant, A. H., Mahony, E. M., Khosla, S. K., Goetsch, L. (2002). Mutational Analysis Reveals a Role for the C Terminus of the Proteasome Subunit Rpt4p in Spindle Pole Body Duplication in Saccharomyces cerevisiae. Genetics 162: 705-720 [Abstract] [Full Text]  
• Cosgrove, A. J., Nieduszynski, C. A., Donaldson, A. D. (2002). Ku complex controls the replication time of DNA in telomere regions. Genes Dev. 16: 2485-2490 [Abstract] [Full Text]  
• Sclafani, R. A., Tecklenburg, M., Pierce, A. (2002). The mcm5-bob1 Bypass of Cdc7p/Dbf4p in DNA Replication Depends on Both Cdk1-Independent and Cdk1-Dependent Steps in Saccharomyces cerevisiae. Genetics 161: 47-57 [Abstract] [Full Text]  
• Sherwood, P. W. (2001). The Yeast Genetics Course at Cold Spring Harbor Laboratory: Thirty Years and Counting. Genetics 157: 1399-1402 [Full Text]  
• Gardner, R. D., Poddar, A., Yellman, C., Tavormina, P. A., Monteagudo, M. C., Burke, D. J. (2001). The Spindle Checkpoint of the Yeast Saccharomyces cerevisiae Requires Kinetochore Function and Maps to the CBF3 Domain. Genetics 157: 1493-1502 [Abstract] [Full Text]  
• Tatebayashi, K., Tani, T., Ikeda, H. (2001). Fission Yeast Mog1p Homologue, Which Interacts With the Small GTPase Ran, Is Required for Mitosis-to-Interphase Transition and poly(A)+ RNA Metabolism. Genetics 157: 1513-1522 [Abstract] [Full Text]  
• Yesilaltay, A., Jenness, D. D. (2000). Homo-oligomeric Complexes of the Yeast alpha -Factor Pheromone Receptor Are Functional Units of Endocytosis. Mol. Biol. Cell 11: 2873-2884 [Abstract] [Full Text]  
• Schlesinger, M. B., Formosa, T. (2000). POB3 Is Required for Both Transcription and Replication in the Yeast Saccharomyces cerevisiae. Genetics 155: 1593-1606 [Abstract] [Full Text]  
• Suzuki, T., Park, H., Hollingsworth, N. M., Sternglanz, R., Lennarz, W. J. (2000). PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-glycanase. J. Cell Biol. 149: 1039-1052 [Abstract] [Full Text]  
• Pauling, M. H., McPheeters, D. S., Ares, M. Jr. (2000). Functional Cus1p Is Found with Hsh155p in a Multiprotein Splicing Factor Associated with U2 snRNA. Mol. Cell. Biol. 20: 2176-2185 [Abstract] [Full Text]  
• Hirschman, J. E., Jenness, D. D. (1999). Dual Lipid Modification of the Yeast Ggamma Subunit Ste18p Determines Membrane Localization of Gbeta gamma. Mol. Cell. Biol. 19: 7705-7711 [Abstract] [Full Text]  
• Kim, D.-H., Edwalds-Gilbert, G., Ren, C., Lin, R.-J. (1999). A Mutation in a Methionine tRNA Gene Suppresses the prp2-1 Ts Mutation and Causes a Pre-mRNA Splicing Defect in Saccharomyces cerevisiae. Genetics 153: 1105-1115 [Abstract] [Full Text]  
• Moy, T. I., Silver, P. A. (1999). Nuclear export of the small ribosomal subunit requires the Ran-GTPase cycle and certain nucleoporins. Genes Dev. 13: 2118-2133 [Abstract] [Full Text]  
• Formosa, T., Nittis, T. (1999). Dna2 Mutants Reveal Interactions with Dna Polymerase {alpha} and Ctf4, a Pol {alpha} Accessory Factor, and Show That Full Dna2 Helicase Activity Is Not Essential for Growth. Genetics 151: 1459-1470 [Abstract] [Full Text]  
• Dohrmann, P. R., Oshiro, G., Tecklenburg, M., Sclafani, R. A. (1999). RAD53 Regulates DBF4 Independently of Checkpoint Function in Saccharomyces cerevisiae. Genetics 151: 965-977 [Abstract] [Full Text]  
• Oki, M., Nishimoto, T. (1998). A protein required for nuclear-protein import, Mog1p, directly interacts with GTP-Gsp1p, the Saccharomyces cerevisiae Ran homologue. Proc. Natl. Acad. Sci. USA 95: 15388-15393 [Abstract] [Full Text]  
• Paulovich, A. G., Armour, C. D., Hartwell, L. H. (1998). The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 Genes Are Required for Tolerating Irreparable, Ultraviolet-Induced DNA Damage. Genetics 150: 75-93 [Abstract] [Full Text]  
• Gravel, S., Larrivée, M., Labrecque, P., Wellinger, R. J. (1998). Yeast Ku as a Regulator of Chromosomal DNA End Structure. Science 280: 741-744 [Abstract] [Full Text]  
• Tavormina, P. A., Burke, D. J. (1998). Cell Cycle Arrest in cdc20 Mutants of Saccharomyces cerevisiae Is Independent of Ndc10p and Kinetochore Function but Requires a Subset of Spindle Checkpoint Genes. Genetics 148: 1701-1713 [Abstract] [Full Text]  
• Schutz, A. R., Winey, M. (1998). New Alleles of the Yeast MPS1 Gene Reveal Multiple Requirements in Spindle Pole Body Duplication. Mol. Biol. Cell 9: 759-774 [Abstract] [Full Text]  
• Taura, T., Schlenstedt, G., Silver, P. A. (1997). Yrb2p Is a Nuclear Protein That Interacts with Prp20p, a Yeast Rcc1 Homologue. J. Biol. Chem. 272: 31877-31884 [Abstract] [Full Text]  
• Haberland, J., Becker, J., Gerke, V. (1997). The Acidic C-terminal Domain of rna1p Is Required for the Binding of Ran·GTP and for RanGAP Activity. J. Biol. Chem. 272: 24717-24726 [Abstract] [Full Text]  
• McDonald, H. B., Byers, B. (1997). A Proteasome Cap Subunit Required for Spindle Pole Body Duplication in Yeast. J. Cell Biol. 137: 539-553 [Abstract] [Full Text]  
• Hirschman, J. E., De Zutter, G. S., Simonds, W. F., Jenness, D. D. (1997). The Gbeta gamma Complex of the Yeast Pheromone Response Pathway. SUBCELLULAR FRACTIONATION AND PROTEIN-PROTEIN INTERACTIONS. J. Biol. Chem. 272: 240-248 [Abstract] [Full Text]  
• Saavedra, C, Tung, K S, Amberg, D C, Hopper, A K, Cole, C N (1996). Regulation of mRNA export in response to stress in Saccharomyces cerevisiae.. Genes Dev. 10: 1608-1620 [Abstract]  
• Demianova, M., Formosa, T. G., Ellis, S. R. (1996). Yeast Proteins Related to the p40/Laminin Receptor Precursor Are Essential Components of the 40 S Ribosomal Subunit. J. Biol. Chem. 271: 11383-11391 [Abstract] [Full Text]  
• Kleff, S., Andrulis, E. D., Anderson, C. W., Sternglanz, R. (1995). Identification of a Gene Encoding a Yeast Histone H4 Acetyltransferase. J. Biol. Chem. 270: 24674-24677 [Abstract] [Full Text]  
• Becker, Jör., Melchior, F., Gerke, V., Bischoff, F. R., Ponstingl, H., Wittinghofer, A. (1995). RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae. J. Biol. Chem. 270: 11860-11865 [Abstract] [Full Text]  
• Danaie, P., Wittmer, B., Altmann, M., Trachsel, H. (1995). Isolation of a Protein Complex Containing Translation Initiation Factor Prt1 from Saccharomyces cerevisiae. J. Biol. Chem. 270: 4288-4292 [Abstract] [Full Text]  
• Singleton, D., Chen, S, Hitomi, M, Kumagai, C, Tartakoff, A. (1995). A yeast protein that bidirectionally affects nucleocytoplasmic transport. J. Cell Sci. 108: 265-272 [Abstract]  
• Creutz, C. E., Kambouris, N. G., Snyder, S. L., Hamman, H. C., Nelson, M. R., Liu, W., Rock, P. (1992). Effects of the expression of mammalian annexins in yeast secretory mutants. J. Cell Sci. 103: 1177-1192 [Abstract]  
• Forrester, W, Stutz, F, Rosbash, M, Wickens, M (1992). Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure.. Genes Dev. 6: 1914-1926 [Abstract]  
• Jones, B A, Fangman, W L (1992). Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin.. Genes Dev. 6: 380-389 [Abstract]  
• Shannon, K W, Guthrie, C (1991). Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.. Genes Dev. 5: 773-785 [Abstract]  
• Strauss, E J, Guthrie, C (1991). A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.. Genes Dev. 5: 629-641 [Abstract]  
• Lustig, A., Kurtz, S, Shore, D (1990). Involvement of the silencer and UAS binding protein RAP1 in regulation of telomere length. Science 250: 549-553 [Abstract]  
• Moran, L, Norris, D, Osley, M A (1990). A yeast H2A-H2B promoter can be regulated by changes in histone gene copy number.. Genes Dev. 4: 752-763 [Abstract]  
• Vijayraghavan, U, Company, M, Abelson, J (1989). Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.. Genes Dev. 3: 1206-1216 [Abstract]  
• Weinert, T., Hartwell, L. (1988). The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241: 317-322 [Abstract]  
• Lin, R J, Lustig, A J, Abelson, J (1987). Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors.. Genes Dev. 1: 7-18 [Abstract]  
• Shapiro, L., Agabian-Keshishian, N., Bendis, I. (1971). Bacterial Differentiation. Science 173: 884-892 [Abstract]