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Measurement of Binding of Chloramphenicol by Intact Cells

¹ Basic Science Research Laboratory, Veterans Administration Hospital, and Department of Microbiology, Albany Medical College, Albany, New York 12208

ABSTRACT

The binding of chloramphenicol to intracellular components of intact cells was measured by procedures based on a silicone-wash technique. The number of stereospecifically bound molecules of chloramphenicol increased with external concentration to a saturation value equal to the number of ribosomes per cell. Chloramphenicol is therefore believed to be attached stereospecifically by a weak bond, most probably to a single site on the 50S ribosome. This bond was found to be temperature-dependent and appeared to be responsible for inhibition of protein synthesis.