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Occurrence of 3-Amino-3,6-Dideoxyhexoses in Salmonella and Related Bacteria

Max Planck Institut für Immunbiologie, Freiburg, Germany
Departments of Microbiology and Biochemistry, Duke University Medical Center, Durham, North Carolina 27706

ABSTRACT

Several species of Salmonella, Citrobacter, and Arizona were examined for the presence of 3-amino sugars, which were isolated from lipopolysaccharide hydrolysates by cation exchange chromatography and identified by paper and cation exchange chromatography in several systems and by specific colorimetric procedures. 3-Amino-3,6-dideoxyglucose was identified in C. freundii 8090, C. freundii 869, S. halle, S. telaviv, S. dakar, S. wandsworth, and S. champaign; 3-amino-3,6-dideoxygalactose was found in S. tranoroa and Arizona 24. Evidence suggests that these 3-amino sugars occur in lipopolysaccharides as the N-acetyl derivatives. The composition of the lipopolysaccharides containing the 3-amino sugars was determined, and the lipopolysaccharides were compared serologically in order to correlate chemotypes with serotypes. The lipopolysaccharides containing 3-amino-3,6-dideoxyglucose reacted with serogroups 28 or 39; those containing 3-amino-3,6-dideoxygalactose were found to react with group 55 antisera. The preparation of a lipopolysaccharide from the phenol phase of the 44% aqueous phenol extraction procedure is also reported.