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Effect of Glucose on the Oxidation of ß-Nitropropionic Acid by Aspergillus flavus

¹ Department of Microbiology, The University of Iowa, Iowa City, Iowa 52241

ABSTRACT

The rate of oxidation of ß-nitropropionic acid (BNP) to nitrate by replacement cultures of Aspergillus flavus was linear. For each micromole of BNP assimilated, 1 µmole of nitrate was detected. BNP was readily oxidized at pH 3.8, but not at pH 6.8. In the presence of glucose, nitrate was not detected until after all of the glucose had been utilized. The effect of glucose is attributed to its inhibition of BNP uptake and also to its role as an electron donor for a nitrate reductase.