This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Murphy, S. G. Search for Related Content PubMed PubMed Citation Articles by Murphy, S. G.

Tetanus Toxin and Antigenic Derivatives II. Effect of Protein and Formaldehyde Concentration on Toxoid Formation

¹ Division of Laboratories and Research, New York State Department of Health, and Department of Microbiology, Albany Medical College, Albany, New York 12201

ABSTRACT

Tetanus toxoid formation was examined under varying conditions. Products of the reaction depended upon the concentration of both formaldehyde and toxin. High concentrations of protein and formaldehyde favored the formation of large polymers, whereas low concentrations yielded smaller polymers and monomer. The monomer had an observed S value of 7.1, whereas the polymers ranged from 10.1S to 110S. The cross-linking between toxin molecules to form toxoid polymers appeared to be random.