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J Bacteriol. 1967 October; 94(4): 1150-1153
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Microcysts of the Cellular Slime Mold Polysphondylium pallidum II. Chemistry of the Microcyst Walls¹

^a Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Methods are described for obtaining large masses of myxamoebae, for inducing these to form microcysts, and for the isolation of the microcyst walls from other cell components. The walls were fractionated into two parts, one alkali-soluble, the other alkali insoluble. The alkali-insoluble fraction is a type of cellulose and constitutes 28% of the microcyst wall by weight. The alkali-soluble fraction contains a glycogenlike material, lipids, and proteins. A possible mechanism of microcyst wall synthesis is discussed.

² Present address: Faculty of Pharmacy, University of Cairo, United Arab Republic.

¹ Part of a thesis offered by the senior author in partial fulfillment of the requirements for the Ph.D. degree, University of Wisconsin.