JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arbuthnott, J. P.
Right arrow Articles by Bernheimer, A. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arbuthnott, J. P.
Right arrow Articles by Bernheimer, A. W.

 Previous Article  |  Next Article 

J Bacteriol. 1967 October; 94(4): 1170-1177
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Physical States of Staphylococcal {alpha}-Toxin

John P. Arbuthnott, John H. Freer and Alan W. Bernheimer

1 Department of Microbiology, New York University School of Medicine, New York, New York 10016

ABSTRACT

At least three different forms of staphylococcal {alpha}-toxin have been shown to exist: soluble active {alpha}-toxin ({alpha} 3S), soluble inactive {alpha}-toxin ({alpha}12s), and insoluble inactive aggregate. Aggregation to the insoluble, biologically inactive form could be induced by brief heating to 60 C. The aggregate was dissociated by treatment with 8 M urea with reappearance of biological activity. Subsequent removal of urea by dialysis resulted in some spontaneous reaggregation to the insoluble state. The supernatant fluid obtained after dialysis contained soluble active {alpha}-toxin of high specific activity, possessing physical, toxic, and immunological properties closely resembling those of native toxin. The soluble biologically inert component ({alpha}12s) was identified as a third physical state. Negatively stained preparations of this material, when examined in the electron microscope, showed rings of approximately 100 A outside diameter containing 6 ± 1 subunits.

J Bacteriol. 1967 October; 94(4): 1170-1177
Copyright © 1967 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 1967 by the American Society for Microbiology. All rights reserved.