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In Vivo and In Vitro Manifestations of Adrenergic Blockade in Bordetella pertussis-vaccinated Mice¹

^a Department of Microbiology, University of Louisville School of Medicine, Louisville, Kentucky 40202

ABSTRACT

The in vivo metabolic events which follow the administration of epinephrine, norepinephrine, or isoproterenol were examined in normal, Bordetella pertussis-vaccinated, and and ß adrenergically blocked mice. The normal hyperglycemic response to epinephrine was suppressed in all experimental groups. The pertussis-sensitized and ß-blocked animals produced similar split patterns of altered response not duplicated by the -blocking compounds. Those catecholamines that normally increase free fatty acids and lactic acid in the circulation failed to do so in the pertussis-sensitized and ß-blocked animals; the inhibition of free fatty acid mobilization was also demonstrated with adipose tissue incubated in vitro. An extract of the pertussis organism added to incubation media prevented the catecholamine-induced free fatty acid response. The epinephrine-stabilizing effect of bovine serum albumin (Cohn-fraction V) was observed. The results of these studies further emphasize a correlation between pertussis-sensitized and ß-adrenergically blocked mice.

¹ Part of the material in this paper was taken from a thesis submitted by the senior author to the Graduate School of the University of Louisville in partial fulfillment of the requirements for the Ph.D. degree.