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Effect of Some Proteins on the Yeast Cell Membrane

^a Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439

ABSTRACT

Yeast cells, Candida utilis, in water suspension and in the absence of electrolytes were found to be very sensitive to several proteins of moderate size, including ribonuclease, protamine, lysozyme, bovine serum albumin, cytochrome c, and myoglobin. Viability ceases rapidly, and ultraviolet-absorbing compounds (260 mµ) and the amino acid pool are released into the medium. The ultraviolet-absorbing material appears to be the nucleotide and coenzyme fraction usually extracted by 0.2 N perchloric acid at low temperature. The ribonucleic acid fraction remains in the cell ghosts and can be released by ribonuclease. The enzymatic properties of some of these proteins have no relation to their damaging effect on the cell membrane. Poly-L-lysine shows the same activity.

¹ Guest investigator, 1966; permanent address: Department of Biology, State University of New York at Buffalo 14214.

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