This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Elbein, A. D. Search for Related Content PubMed PubMed Citation Articles by Elbein, A. D.

Carbohydrate Metabolism in Streptomycetes II. Isolation and Enzymatic Synthesis of Trehalose¹

^a Department of Biology, Rice University, Houston, Texas 77001

ABSTRACT

A number of streptomycetes were examined for their ability to synthesize trehalose phosphate as well as for the presence of ,-trehalose. In each case, an enzyme system was demonstrated which catalyzed the transfer of glucose from guanosine diphosphate-glucose to glucose-6-phosphate to form trehalose phosphate. Thus, this group of organisms appears to synthesize trehalose phosphate by a different mechanism from that described in insects, yeast, and fungi. In addition, trehalose was isolated from each of these organisms. In several of these cases, crystallization of the sugar and determination of the physical properties showed that the sugar was ,-trehalose.

² Recipient of a Research Career Development Award from the National Institute of Allergy and Infectious Diseases.

¹ A preliminary report covering part of this work was presented at the 67th Annual Meeting of the American Society for Microbiology, New York, N.Y.