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J Bacteriol. 1967 December; 94(6): 1930-1933
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Biosynthesis of Biotin in Microorganisms VI. Further Evidence for Desthiobiotin as a Precursor in Escherichia coli

Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada
College of Medicine, University of Cincinnati, Cincinnati, Ohio 45219

ABSTRACT

Biotin auxotrophs were isolated from Escherichia coli K-12. One of the mutants was unable to grow on desthiobiotin and accumulated a large amount of a vitamer in medium when growing on an optimal concentration of biotin. The production of the vitamer was inhibited in the presence of an excess amount of biotin. The vitamer was identified as desthiobiotin on the basis of biological activities, avidin combinability, and chromatographic characteristics. The mutant lacked the ability to convert desthiobiotin to biotin. These results further support the hypothesis that desthiobiotin is a normal intermediate in the biosynthesis of biotin in E. coli.