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J Bacteriol. 1968 January; 95(1): 147-151
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Inhibition of Oxygen Utilization and Destruction of Ubiquinone by Ultraviolet Irradiation of Thiobacillus thiooxidans¹

^a Department of Microbiology, Rutgers, The State University, New Brunswick, New Jersey 08903

ABSTRACT

Ultraviolet (UV) irradiation inhibited sulfur oxidation by cells of Thiobacillus thiooxidans. Sulfur-oxidizing activity decreased as the exposure time to UV light increased. A loss of the ability of cells of fix CO₂ paralleled the loss of sulfur-oxidizing activity. UV light photoinactivated ubiquinone purified from T. thiooxidans. The same percentage of sulfur-oxidizing activity and ubiquinone was destroyed after 15 min of UV exposure. Both the photoinactivation of sulfur oxidation and ubiquinone followed first-order reaction kinetics. The specific rate constants for both photoinactivations were nearly equal. Cells completely inactivated by UV light contained no ubiquinone. Ubiquinone was found to be a component of the cell wall-membrane complex.

² Present address: Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822.

¹ Presented in part at the 67th Annual Meeting of the American Society for Microbiology, New York, N.Y., 1967.