This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Stokes, J. L. Articles by Larkin, J. M. Search for Related Content PubMed PubMed Citation Articles by Stokes, J. L. Articles by Larkin, J. M.

 Previous Article  |  Next Article 

J Bacteriol. 1968 January; 95(1): 95-98
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Comparative Effect of Temperature on the Oxidative Metabolism of Whole and Disrupted Cells of a Psychrophilic and a Mesophilic Species of Bacillus

¹ Department of Bacteriology and Public Health, Washington State University, Pullman, Washington 99163

ABSTRACT

We investigated the influence of temperature, in the range of 45 to 5 C, on the rate of oxidation of glucose and citrate by intact cells and cell-free extracts of psychrophilic Bacillus psychrophilus and mesophilic B. thuringiensis. Both glucose and endogenous oxidation by whole and disrupted cells of the psychrophile decreased more slowly with decrease in temperature than did glucose and endogenous oxidation by whole and disrupted cells of the mesophile. Similar results were obtained for the oxidation of citrate by cell-free extracts. Since substrate permeability is not involved in the oxidative metabolism of the cell-free extracts, we concluded that the internal enzymes of the psychrophile differ from those of the mesophile.