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J Bacteriol. 1968 March; 95(3): 1118-1123
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Hydrogen-oxidizing Methane Bacteria I. Cultivation and Methanogenesis

¹ Departments of Microbiology and Dairy Science, University of Illinois, Urbana, Illinois 61801

ABSTRACT

A method for the mass culture of hydrogen-oxidizing methane bacteria has been developed; yields of 50 to 60 g (wet weight) of cells per 12 to 14 liters of culture medium were obtained. The methanogenic organism from the culture of Methanobacillus omelianskii was grown in a complex medium which was aerated with a gas mixture of hydrogen and carbon dioxide. Extracts prepared from hydrogen-grown cells formed methane from methyl cobalamin, 5-methyl tetrahydrofolate, serine, pyruvate, and carbon dioxide; these substrates have been shown to be precursors of methane in extracts of the ethyl alcohol-grown culture of M. omelianskii.