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J Bacteriol. 1968 March; 95(3): 824-832
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Regulation of Pyrimidine Biosynthesis in Saccharomyces cerevisiae¹

^a Laboratoire de Génétique Physiologique, Centre National de la Recherche Scientifique, Gif-sur-Yvette, Essonne, France

ABSTRACT

Biochemical steps of the pyrimidine pathway have been found to be the same in yeast as in bacteria, and all except one step have been characterized. The activities of the first two enzymes, carbamoyl phosphate synthetase and aspartic transcarbamylase, are simultaneously controlled by feedback inhibition and repression. Moreover, these enzymes are coded by the same genetic region (ura-2) and seem to form a single enzymatic complex. The enzymes that follow later in the pathway are induced in a sequential way by the intermediary products and are insensitive to pyrimidine repression. The corresponding genes (ura-4, ura-1, ura-3) are not linked to each other or to ura-2, the gene for carbamoyl phosphate synthetase and aspartic transcarbamylase. Mutants that have simultaneously lost feedback inhibition by uridine triphosphate for carbamoyl phosphate synthetase and for aspartic transcarbamylase have been found and mapped in the gene ura-2.

² Present address: Laboratoire de Génétique Physiologique, Institut de Botanique, 8 rue Goethe, 67 Strasbourg, France.

¹ Preliminary reports of this work have been published (11, 12, 14).

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