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J Bacteriol. 1968 March; 95(3): 901-906
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Murine Toxicity of Agrobacterium tumefaciens1

Pat B. Hamilton2,a and Donald Huisinghb

a Research Triangle Institute, Research Triangle Park, North Carolina
Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27607

ABSTRACT

Eleven strains of the crown gall organism, Agrobacterium tumefaciens, tested by intraperitoneal injection into mice, were lethal within 48 hr. Five other species had some lethal strains. The lethal effect of A. tumefaciens appeared to be the result of a toxic rather than an infectious process, since histopathological anomalies were not found in mice injected with live cultures and since heat-killed cultures were lethal. The murine toxin disappeared when A. tumefaciens was grown at 36 C and reappeared when the organism was subsequently incubated below 30 C. The murine toxin itself was not inactivated by exposure to 100 C for 30 min. The toxin was associated with the cells and was not excreted into the medium. Centrifugal fractionation revealed that the toxin was associated with the smaller cells in 3-day stationary-phase cultures. These data suggested a possible relationship between toxin production and the production of the agents responsible for the initiation of plant tumors.

FOOTNOTES

2 Present address: Department of Poultry Science, North Carolina State University, Raleigh, N.C. 27607.

1 Paper no. 2430 of the Journal Series of the North Carolina State University Agricultural Experiment Station, Raleigh.

J Bacteriol. 1968 March; 95(3): 901-906
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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