This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hufham, J. B. Articles by Pfiffner, J. J. Search for Related Content PubMed PubMed Citation Articles by Hufham, J. B. Articles by Pfiffner, J. J.

 Previous Article  |  Next Article 

J Bacteriol. 1968 March; 95(3): 947-951
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Microbial Degradation of Corrinoids VI. Reduction of Hydroxocobalamin by Cell-free Particles from Pseudomonas rubescens

^a Department of Physiology and Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48207

ABSTRACT

Cell-free particles from Pseudomonas rubescens have been shown to reduce hydroxocobalamin to vitamin B_12r. The particles are unable to reduce the B_12r to B_12s. The reduction of hydroxocobalamin is dependent upon reduced nicotinamide adenine dinucleotide and is stimulated by flavin adenine dinucleotide. Cobinamide and diaquocobinamide were reduced at 25 and 10%, respectively, of the rate of hydroxocobalamin. Cyanocobalamin, coenzyme B₁₂, pseudovitamin B₁₂, and diaquopseudocobalamin were not reduced. Reduced nicotinamide adenine dinucleotide phosphate and flavin mononucleotide were not active. Diaphorase and xanthine oxidase activity were not present in the particulate fraction.

¹ Present address: Space Defense Corp., Birmingham, Mich. 48011.

² Present address: Department of Physiology, Baylor University College of Medicine, Houston, Tex. 77025.