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J Bacteriol. 1968 March; 95(3): 980-982
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Quantitative Assay of Polysaccharide Components Obtained from Cell Wall Lipopolysaccharides of Xanthomonas Species

Department of Microbiology, School of Medicine, University of Virginia, Charlottesville, Virginia 22903

ABSTRACT

The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all preparations contained uronic acid, phosphate, and mannose in molar ratios of approximately 1:2:1, and glucose and rhamnose in more variable concentrations. Most lipopolysaccharides contained either xylose or fucose, but those extracted from X. sinensis and X. campestris contained neither xylose nor fucose.