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J Bacteriol. 1968 April; 95(4): 1221-1237
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Nuclear Fraction of Bacillus subtilis as a Template for Ribonucleic Acid Synthesis

S. Mizuno1 and H. R. Whiteley2

a Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98105

ABSTRACT

A "nuclear fraction" prepared from Bacillus subtilis was a more efficient template than purified deoxyribonucleic acid for the synthesis of ribonucleic acid by exogenously added ribonucleic acid polymerase isolated from B. subtilis. The initial rate of synthesis with the nuclear fraction was higher and synthesis continued for several hours, yielding an amount of ribonucleic acid greater than the amount of deoxyribonucleic acid used as the template. The product was heterogenous in size, with a large portion exceeding 23S. When purified deoxyribonucleic acid was the template, a more limited synthesis was observed with a predominantly 7S product. However, the ribonucleic acids produced in vitro from these templates were very similar to each other and to in vivo synthesized ribonucleic acid as determined by the competition of ribonucleic acid from whole cells in the annealing of in vitro synthesized ribonucleic acids to deoxyribonucleic acid. Treatment of the nuclear fraction with heat (60 C for 15 min) or trypsin reduced the capacity of the nuclear fraction to synthesize ribonucleic acid to the level observed with purified deoxyribonucleic acid.

FOOTNOTES

1 Present address: Laboratory of Radiation Genetics, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo, Japan.

2 Recipient of Research Career Award GM-K6-422.

J Bacteriol. 1968 April; 95(4): 1221-1237
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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