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J Bacteriol. 1968 April; 95(4): 1272-1278
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Genetic Mapping of Loci for Glucose-6-Phosphate Dehydrogenase, Gluconate-6-Phosphate Dehydrogenase, and Gluconate-6-Phosphate Dehydrase in Escherichia coli

Graciela Peyru and D. G. Fraenkel

1 Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT

The loci on the Escherichia coli genome of mutations affecting the constitutive enzymes glucose-6-phosphate dehydrogenase (zwf) and gluconate-6-phosphate dehydrogenase (gnd), and the inducible enzyme gluconate-6-phosphate dehydrase (edd), were determined by conjugation and transduction experiments, chiefly by three-factor crosses. They are in the same region of the chromosome, and their order is gnd—his—(edd, zwf)—aroD; gnd and his are cotransduceable, as are zwf and edd. The position of gnd in Salmonella typhimurium was shown to be similar to that in E. coli.


J Bacteriol. 1968 April; 95(4): 1272-1278
Copyright © 1968 American Society for Microbiology. All Rights Reserved.







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