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J Bacteriol. 1968 April; 95(4): 1431-1438
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Analysis of Sporulation Mutants II. Mutants Blocked in the Citric Acid Cycle

Peter Fortnagel1 and Ernst Freese

a Laboratory of Molecular Biology, National Institute of Neurological Diseases and Blindness, Bethesda, Maryland 20014

ABSTRACT

Sporulation mutants that were unable to incorporate uracil during the developmental period recovered this capacity with the addition of ribose and in most cases with the addition of glutamate. Of the mutants that responded to both ribose and glumate, all but three also responded to citrate, and all but five responded to acetate. One of the exceptional strains was deficient in aconitase and another one in aconitase and isocitrate dehydrogenase; both required glutamate for growth. For the mutants which did not respond to glutamate, the products made from 14C-glutamate were determined by thin-layer chromatography. Significant differences were found which enabled the identification of mutant blocks. The deficiency of the corresponding enzyme activity was verified. Several mutants were deficient in {alpha}-ketoglutarate dehydrogenase, and one lacked succinic dehydrogenase. These mutants could still grow on glucose as sole carbon source, but not on glutamate. The intact Krebs cycle is therefore not required for vegetative growth of aerobic Bacillis subtilis, but it is indispensable for sporulation.

FOOTNOTES

1 Fellow of The Anna Fuller Fund, New Haven, Conn.

J Bacteriol. 1968 April; 95(4): 1431-1438
Copyright © 1968 American Society for Microbiology. All Rights Reserved.



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